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KMID : 0881720200350060561
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Journal of Food Hygiene and Safety
2020 Volume.35 No. 6 p.561 ~ p.566
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Development and Validation of Multiplex PCR Method for the Identification of Pagrus major and Sciaenops ocellatus
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Choi I-Seul
Shin Ji-Young
Yang Ji-Young
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Abstract
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Nowadays, with increase of seafood consumption, there have been increasing reports of defectiveseafood products. There have been incidents of red drum (Sciaenops ocellatus) being sold as red seabream (Pagrusmajor). In this study, we sought to develop and validate species-specific PCRs to differentiate between P. major andS. ocellatus to prevent the sale of S. ocellatus as P. major. Primers for P. major were designed to bind 12s rRNA andthose for S. ocellatus were designed to bind 16s rRNA. Multiplex PCR showed a 468 bp amplicon for P. major and a181 bp amplicon for S. ocellatus. The limit of detection of P. major and S. ocellatus was present at 1 ng each. Thedeveloped primers were validated with 19 P. major samples of food items purchased through the internet. Using thismonitoring method, the experimental results and tested species were in agreement. Hence, the developed multiplexPCR method is considered reliable to authenticate P. major and S. ocellatus.
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KEYWORD
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Pagrus major, Sciaenops ocellatus, Multiplex PCR
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